In-vitro ANTICANCER ACTIVITY OF LICHEN Heterodermia boryi AND ITS SECONDARY METABOLITES

Lichens are symbiotic organisms that have been traditionally used for treating different kinds of ailments. Heterodermia boryi is a common foliose lichen found growing luxuriantly in the Western Ghats. It clear from literature survey there no reports on isolation and biological activity substances boryi, we thought exploring anticancer its crude extract isolated compounds. Fractionation methanolic by column chromatography led to three pure The compounds were screened vitro cytotoxic against human cancer cell lines such as breast (MCF-7), lung (H1975), colon (HCT-116). Isolated with highest further evaluated their ability induce apoptosis dual staining method MCF-7 line. Molecular docking studies carried out study interact Mitogen-activated protein kinases- 2 (Mnk2). characterized methyl haematommate (HKB-1), atraric acid (HKB-4), zeorin (HKB-6) spectral methods. Among compounds, HKB-1 HKB-6 exhibited superior IC50 values 11.1 µg/ml 17.02 respectively. Dual cells treated at 10 displayed early signs apoptosis. revealed excellent binding affinity interaction site residues Mnk2 comparable co-crystallized ligand staurosporine indicative possible role inhibitors.